Silicon induces adventitious root formation in rice under arsenate stress with involvement of nitric oxide and indole-3-acetic acid

Abstract Arsenic (As) negatively affects plant development. This study evaluates how the application of silicon (Si) can favor formation adventitious roots in rice under arsenate stress (AsV) as a mechanism to mitigate its negative effects. The simultaneous AsV and Si up-regulated expression genes involved nitric oxide (NO) metabolism, cell cycle progression, auxin (IAA, indole-3-acetic acid) biosynthesis transport, uptake which accompanied root formation. Furthermore, triggered activity enzymes ascorbate recycling. Treatment with L-NAME (NG-nitro L-arginine methyl ester), an inhibitor NO generation, significantly suppressed formation, even presence Si; however, supplying growth media rescued Our data suggest that both IAA are essential for Si-mediated stress. Interestingly, TIBA (2,3,5-triiodobenzoic acid), polar transport inhibitor, SNP (sodium nitroprusside, donor), suggesting is whereby cellular signal first requires followed by biosynthesis.